|
|
|
|
|
| Queiroz,Paulo Roberto; Martins,Erica Soares; Klautau,Nazaré; Lima,Luzia; Praça,Lilian; Monnerat,Rose Gomes. |
| Abstract: The objective of this work was to develop sequence-characterized amplified region (Scar) markers to identify the B, Q, and native Brazilian biotypes of the sweet potato whitefly [Bemisia tabaci (Hemiptera: Aleyrodidae)]. Random amplified polymorphic DNA (RAPD) amplification products, exclusive to the B and Brazilian biotypes, were selected after the analysis of 12,000 samples, in order to design a specific Scar primer set. The BT-B1 and BT-B3 Scar markers, used to detect the B biotype, produced PCR fragments of 850 and 582 bp, respectively. The BT-BR1 Scar marker, used to identify the Brazilian biotype, produced a PCR fragment of 700 bp. The Scar markers were tested against the Q biotype, and a flowchart was proposed to indicate the decision... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aleyrodidae; Commodities; Field samples; Molecular marker; Sweet potato whitefly.. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2016000500555 |
| |
|
|
| Monnerat,Rose Gomes; Dias,Daniel Gerhein Souza; Silva,Silvania Ferreira da; Martins,Erica Soares; Berry,Colin; Falcão,Rosana; Gomes,Ana Cristina Menezes Mendes; Praça,Lilian Botelho; Soares,Carlos Marcelo Silveira. |
| The objective of this work was to evaluate 210 Bacillus thuringiensis strains against Aedes aegypti and Culex quinquefasciatus larvae to select the most effective. These strains were isolated from different regions of Brazil and are stored in a Bacillus spp. collection at Embrapa Recursos Genéticos e Biotecnologia, Brasília, Brazil. The selected strains were characterized by morphological (microscopy), biochemical (SDS-PAGE 10%) and molecular (PCR) methods. Six B. thuringiensis strains were identified as mosquito-toxic after the selective bioassays. None of the strains produced the expected PCR products for detection of cry4, cry11 and cyt1A genes. These results indicate that the activity of mosquitocidal Brazilian strains are not related with Cry4, Cry11... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Culex quinquefasciatus; Aedes aegypti; Bioinsecticide. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2005000200001 |
| |
|
|
|
